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il 1β concentration  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Multi Sciences (Lianke) Biotech Co Ltd il 1β concentration
    Il 1β Concentration, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/il+1%CE%B2+concentration/Rat+IL-1%CE%B2+Standard/pm39838454-99-10-21
    Average 95 stars, based on 19 article reviews
    il 1β concentration - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: TREM2 promotes the formation of a tumor-supportive microenvironment in hepatocellular carcinoma.
    Article Snippet: .. The cell culture supernatant was diluted 30 times, and the IL-1β concentration was assessed using the Mouse IL-1β ELISA Kit (EK201B, MULTISCIENCES) according to the manufacturer’s instructions. ..

    Article Title: TREM2 promotes the formation of a tumor-supportive microenvironment in hepatocellular carcinoma
    Article Snippet: .. The cell culture supernatant was diluted 30 times, and the IL-1β concentration was assessed using the Mouse IL-1β ELISA Kit (EK201B, MULTISCIENCES) according to the manufacturer’s instructions. ..

    Concentration Assay:

    Article Title: TREM2 promotes the formation of a tumor-supportive microenvironment in hepatocellular carcinoma.
    Article Snippet: .. The cell culture supernatant was diluted 30 times, and the IL-1β concentration was assessed using the Mouse IL-1β ELISA Kit (EK201B, MULTISCIENCES) according to the manufacturer’s instructions. ..

    Article Title: Echinococcus granulosus cyst fluid inhibits KDM6B-mediated demethylation of trimethylated histone H3 lysine 27 and interleukin-1β production in macrophages.
    Article Snippet: Enzyme‐linked immunosorbent assays Cell culture medium was collected and centrifuged at 300 g for 10 min, and the supernatant was collected. .. IL-1β concentration was determined by using a human IL-1β enzyme-linked immunosorbent assay (ELISA) kit (EK101B; Multi Sciences) and mouse IL-1β ELISA Kit (EK201B; Multi Sciences), in accordance with the manufacturer’s instructions, and the optical density at 450 nm (wavelength correction at 570 nm) was measured using a Varioskan LUX Multimode Microplate Reader (Thermo Scientific). .. Intracellular levels of ROS were measured by using the Reactive oxygen species Assay Kit (Nanjing Jiancheng Bioengineering Institute) in strict accordance with the manufacturer’s instructions.

    Article Title: TREM2 promotes the formation of a tumor-supportive microenvironment in hepatocellular carcinoma
    Article Snippet: .. The cell culture supernatant was diluted 30 times, and the IL-1β concentration was assessed using the Mouse IL-1β ELISA Kit (EK201B, MULTISCIENCES) according to the manufacturer’s instructions. ..

    Article Title: Bone-derived PDGF-BB enhances hippocampal non-specific transcytosis through microglia-endothelial crosstalk in HFD-induced metabolic syndrome
    Article Snippet: HBMECs were treated with IL-1β (10 ng/mL, 10,139-HNAE, Sino Biological) or combined with ALPL inhibitor (SBI-425, 50 μM, HY-124,756, MCE) for 1 d and harvested for further analysis. .. Hundred microliters of ′CM per well was used to detect IL-1β concentration using human IL-1β ELISA kit (70-EK201BHS, Multi Sciences, China) according to manufacturer’s protocol. ..

    Article Title: Bone-derived PDGF-BB enhances hippocampal non-specific transcytosis through microglia-endothelial crosstalk in HFD-induced metabolic syndrome.
    Article Snippet: HBMECs were treated with IL-1β (10 ng/mL, 10,139-HNAE, Sino Biological) or combined with ALPL inhibitor (SBI-425, 50 μM, HY-124,756, MCE) for 1 d and harvested for further analysis. .. Hundred microliters of ′CM per well was used to detect IL-1β concentration using human IL-1β ELISA kit (70-EK201BHS, Multi Sciences, China) according to manufacturer’s protocol. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: TREM2 promotes the formation of a tumor-supportive microenvironment in hepatocellular carcinoma.
    Article Snippet: .. The cell culture supernatant was diluted 30 times, and the IL-1β concentration was assessed using the Mouse IL-1β ELISA Kit (EK201B, MULTISCIENCES) according to the manufacturer’s instructions. ..

    Article Title: Echinococcus granulosus cyst fluid inhibits KDM6B-mediated demethylation of trimethylated histone H3 lysine 27 and interleukin-1β production in macrophages.
    Article Snippet: Enzyme‐linked immunosorbent assays Cell culture medium was collected and centrifuged at 300 g for 10 min, and the supernatant was collected. .. IL-1β concentration was determined by using a human IL-1β enzyme-linked immunosorbent assay (ELISA) kit (EK101B; Multi Sciences) and mouse IL-1β ELISA Kit (EK201B; Multi Sciences), in accordance with the manufacturer’s instructions, and the optical density at 450 nm (wavelength correction at 570 nm) was measured using a Varioskan LUX Multimode Microplate Reader (Thermo Scientific). .. Intracellular levels of ROS were measured by using the Reactive oxygen species Assay Kit (Nanjing Jiancheng Bioengineering Institute) in strict accordance with the manufacturer’s instructions.

    Article Title: TREM2 promotes the formation of a tumor-supportive microenvironment in hepatocellular carcinoma
    Article Snippet: .. The cell culture supernatant was diluted 30 times, and the IL-1β concentration was assessed using the Mouse IL-1β ELISA Kit (EK201B, MULTISCIENCES) according to the manufacturer’s instructions. ..

    Article Title: Bone-derived PDGF-BB enhances hippocampal non-specific transcytosis through microglia-endothelial crosstalk in HFD-induced metabolic syndrome
    Article Snippet: HBMECs were treated with IL-1β (10 ng/mL, 10,139-HNAE, Sino Biological) or combined with ALPL inhibitor (SBI-425, 50 μM, HY-124,756, MCE) for 1 d and harvested for further analysis. .. Hundred microliters of ′CM per well was used to detect IL-1β concentration using human IL-1β ELISA kit (70-EK201BHS, Multi Sciences, China) according to manufacturer’s protocol. ..

    Article Title: Bone-derived PDGF-BB enhances hippocampal non-specific transcytosis through microglia-endothelial crosstalk in HFD-induced metabolic syndrome.
    Article Snippet: HBMECs were treated with IL-1β (10 ng/mL, 10,139-HNAE, Sino Biological) or combined with ALPL inhibitor (SBI-425, 50 μM, HY-124,756, MCE) for 1 d and harvested for further analysis. .. Hundred microliters of ′CM per well was used to detect IL-1β concentration using human IL-1β ELISA kit (70-EK201BHS, Multi Sciences, China) according to manufacturer’s protocol. ..



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    Image Search Results


    The putative α7-selective nicotinic acetylcholine receptor (nAChR) agonists S24795 (S24) and PNU-282987 (PNU) inhibit the BzATP-mediated interleukin (IL)-1β release. Monocytic THP-1 cells and THP-1 cell-derived M1-like macrophages were primed for 5 h with lipopolysaccharide (LPS; 1 µg/ml). The P2X7 receptor agonist BzATP was added for another 40 min to trigger IL-1β release, which was quantified by ELISA (A,C) and by measuring IL-1α/β-equivalent bioactive concentrations using HEK-Blue™ IL-1R cells in a QUANTI-Blue™ assay (B, D) . The BzATP- (100 µM) induced release of IL-1β was investigated in the presence and absence of the nAChR agonists S24 (50 µM) and PNU (10 µM), acetylcholine (ACh, 10 µM) or phosphocholine (PC, 200 µM). The amount of IL-1β released in response to BzATP was calculated by subtracting the IL-1β concentrations of cells treated with LPS alone. In each experiment, the IL-1β concentrations obtained after stimulation with BzATP were set to 100% and all other values were calculated accordingly. Data are presented as individual data points, bars represent median, whiskers percentiles 25 and 75. *p ≤ 0.05, different from LPS-primed cells stimulated with BzATP alone. Friedman test followed by the Wilcoxon signed-rank test.

    Journal: Frontiers in Immunology

    Article Title: Putative α7-selective ligands interact with α9-containing nicotinic acetylcholine receptors and modulate immune functions of human mononuclear phagocytes

    doi: 10.3389/fimmu.2026.1773637

    Figure Lengend Snippet: The putative α7-selective nicotinic acetylcholine receptor (nAChR) agonists S24795 (S24) and PNU-282987 (PNU) inhibit the BzATP-mediated interleukin (IL)-1β release. Monocytic THP-1 cells and THP-1 cell-derived M1-like macrophages were primed for 5 h with lipopolysaccharide (LPS; 1 µg/ml). The P2X7 receptor agonist BzATP was added for another 40 min to trigger IL-1β release, which was quantified by ELISA (A,C) and by measuring IL-1α/β-equivalent bioactive concentrations using HEK-Blue™ IL-1R cells in a QUANTI-Blue™ assay (B, D) . The BzATP- (100 µM) induced release of IL-1β was investigated in the presence and absence of the nAChR agonists S24 (50 µM) and PNU (10 µM), acetylcholine (ACh, 10 µM) or phosphocholine (PC, 200 µM). The amount of IL-1β released in response to BzATP was calculated by subtracting the IL-1β concentrations of cells treated with LPS alone. In each experiment, the IL-1β concentrations obtained after stimulation with BzATP were set to 100% and all other values were calculated accordingly. Data are presented as individual data points, bars represent median, whiskers percentiles 25 and 75. *p ≤ 0.05, different from LPS-primed cells stimulated with BzATP alone. Friedman test followed by the Wilcoxon signed-rank test.

    Article Snippet: IL-1α/β-equivalent bioactive concentrations (pg/ml) were determined by interpolation from a 4-parameter logistic standard curve generated with a serial dilution of recombinant human IL-1β (InvivoGen, Cat# rcyc-hil1b) that was dissolved in FBS-free RPMI 1640 medium and run in parallel with the samples.

    Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay